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Cyclin D3 is essential for intestinal epithelial cell proliferation
Tien C Ko1, Fuming Pan, Hongmiao Sheng
1Department of Surgery, The University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555-0542, USA. tko@utmb.edu
World Journal of Surgery
|April 12, 2002
Summary
Cyclin D3 is essential for intestinal epithelial cell proliferation, enabling cells to transition from G0 to S phase. Its expression is not affected by fasting or refeeding, unlike Cdk4.
Area of Science:
- Cell Biology
- Gastroenterology
- Molecular Biology
Background:
- The intestinal epithelium is a highly proliferative tissue.
- D-type cyclins (including cyclin D3) bind cyclin-dependent kinases (Cdk4/Cdk6) and regulate cell cycle progression.
- Previous studies suggested cyclin D3 might not be essential for gut cell proliferation.
Purpose of the Study:
- To determine if cyclin D3 is required for intestinal epithelial cells to transition from the G0 to the S phase.
- To investigate the regulation of cyclin D3 expression in the rat jejunum during fasting and refeeding.
Main Methods:
- Microinjection of anti-cyclin D3 antiserum into quiescent rat intestinal epithelial cells (IEC-6, RIE-1).
- Assessment of cell cycle progression into the S phase.
- Analysis of cyclin D3 and Cdk4 expression in rat jejunal mucosa after fasting and refeeding protocols.
Main Results:
- Microinjection of anti-cyclin D3 antiserum blocked quiescent intestinal cells from entering the S phase.
- Cyclin D3 levels remained unchanged following fasting and refeeding.
- Cdk4 expression was suppressed by fasting but recovered upon refeeding.
Conclusions:
- Cyclin D3 plays an essential role in intestinal epithelial cell proliferation.
- The expression of cyclin D3 in the intestine is not regulated by dietary restriction (fasting/refeeding).