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Enhanced adenoviral-vector mediated gene transfer using human albumin solder
Arnold D C Rivera1, Charles N Walker, Clifford Bleustein
1Department of Urology, The Children's Hospital of New York, Weill Medical College of Cornell University, New York, New York 10021, USA.
Background And Objective:
Laser tissue welding with human albumin solder (HAS) has been used as an alternative method of wound closure. Adenoviral vectors have been used to introduce various cytokine genes into wounds to accelerate wound closure. In the present study, we were interested in the effect of HAS on adenoviral vector transfer of the beta-galactosidase (beta-gal) gene in vitro and in vivo.
Study Design/Materials And Methods:
3T3 fibroblasts were used to study the effect of HAS on beta-gal gene transfer in vitro. The presence of beta-gal was determined by Western blot, and its activity by a colorimetric assay. A punch biopsy model of wound healing in pigs was used for in vivo experiments.
Results:
HAS increased the efficiency of adenoviral-mediated beta-gal transduction and stabilized the adenovirus at room temperature. HAS protected adenovirus from inactivation by laser, both in vitro and in vivo.
Conclusions:
HAS may stabilize adenoviral vectors to deliver cytokine genes in future wound healing experiments.