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A general method for labeling proteins with 11C.

M G Straatmann, M J Welch

    Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
    |May 1, 1975
    PubMed
    Summary
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    A new method efficiently labels proteins using carbon-11 (11C) and 11C-formaldehyde. This technique offers high yields for various molecules with amino groups, suitable for research applications.

    Area of Science:

    • Biochemistry
    • Radiochemistry
    • Molecular Biology

    Background:

    • Protein labeling is crucial for various biological and medical research applications.
    • Existing methods for incorporating radioisotopes like carbon-11 (11C) into proteins can be complex or yield-limited.

    Purpose of the Study:

    • To develop a novel, efficient method for labeling proteins with 11C.
    • To adapt this method for labeling peptides and other molecules containing free amino groups.

    Main Methods:

    • The method involves reacting proteins with 11C-formaldehyde, derived from 11CO2.
    • The resulting adduct is reduced using sodium borohydride.
    • Purification is achieved via high-speed liquid chromatography or ammonium sulfate precipitation for fibrinogen.

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    Main Results:

    • High yields of 11C-labeled proteins were obtained using a mild reaction.
    • The technique is adaptable to various proteins, peptides, and molecules with free amino groups.
    • The labeling process is stable at pH 8.

    Conclusions:

    • A simple and efficient method for 11C-labeling of proteins and related molecules has been established.
    • This technique offers a valuable tool for researchers in biochemistry and molecular imaging.
    • The method's adaptability and high yields make it suitable for diverse applications.