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Recombinant protein expression for therapeutic applications.

Dana C Andersen1, Lynne Krummen

  • 1Cell Culture & Fermentation Research & Development, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. andersen@gene.com

Current Opinion in Biotechnology
|April 13, 2002
PubMed
Summary

Therapeutic recombinant protein production is growing, especially for complex glycoproteins and antibodies. Advances in mammalian and microbial expression systems are meeting these increased demands.

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Area of Science:

  • Biotechnology
  • Biopharmaceutical Manufacturing
  • Protein Engineering

Background:

  • The demand for therapeutic recombinant proteins, including complex glycoproteins and antibodies, has significantly increased.
  • High production requirements for these biologics necessitate advanced expression technologies.

Purpose of the Study:

  • To highlight advancements in protein expression technology driven by the growing need for therapeutic proteins.
  • To discuss improvements in mammalian and microbial culture systems for recombinant protein production.

Main Methods:

  • Review of recent developments in protein expression systems.
  • Focus on advancements in mammalian cell culture.
  • Focus on advancements in microbial fermentation systems.

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Main Results:

  • Significant improvements in recombinant protein yield and quality have been achieved.
  • Mammalian and microbial systems demonstrate enhanced capabilities for producing complex therapeutic proteins.
  • Expression technologies are adapting to meet high-volume production demands.

Conclusions:

  • Protein expression technology, particularly in mammalian and microbial systems, has advanced considerably.
  • These advancements are crucial for meeting the increasing global demand for therapeutic recombinant proteins.
  • Continued innovation in expression systems will be vital for future biopharmaceutical development.