Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Non-viral gene delivery systems.

Mark E Davis1

  • 1Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. mdavis@cheme.caltech.edu

Current Opinion in Biotechnology
|April 16, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis and Characterization of Silicoaluminophosphate CIT-16P and Its Transformation to SAPO-17.

Inorganic chemistry·2023
Same author

Carbon dioxide capture with zeotype materials.

Chemical Society reviews·2022
Same author

Confinement effects facilitate low-concentration carbon dioxide capture with zeolites.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Structure Elucidation and Computationally Guided Synthesis of SSZ-43: A One-Dimensional 12-Ring Zeolite with Unique Sinusoidal Channels.

Angewandte Chemie (International ed. in English)·2022
Same author

Zinc Containing Small-Pore Zeolites for Capture of Low Concentration Carbon Dioxide.

Angewandte Chemie (International ed. in English)·2021
Same author

β-Cyclodextrin-containing polymer treatment of cutaneous lupus and influenza improves outcomes.

Molecular therapy : the journal of the American Society of Gene Therapy·2021
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Non-viral gene delivery systems using lipids and polymers show promise for nucleic acid pharmaceuticals. Recent advancements address manufacturing, formulation, and in vivo challenges for commercial viability.

Area of Science:

  • Biotechnology
  • Pharmaceutical Sciences
  • Gene Therapy

Background:

  • Non-viral gene delivery systems offer a pathway for developing nucleic acid-based pharmaceuticals.
  • These systems commonly utilize lipids and/or cationic polymers for DNA delivery.

Purpose of the Study:

  • To highlight the potential of non-viral gene delivery systems in pharmaceutical development.
  • To identify and discuss the key barriers hindering the commercialization of these systems.
  • To summarize recent progress in overcoming these identified challenges.

Main Methods:

  • Review of current non-viral gene delivery technologies.
  • Analysis of manufacturing, formulation, and stability challenges.
  • Examination of in vivo barriers, including extracellular interactions and intracellular trafficking.

Related Experiment Videos

Main Results:

  • Non-viral systems face significant hurdles in large-scale manufacturing, precise formulation, and long-term stability.
  • In vivo administration encounters issues with non-specific interactions outside cells and inefficient transport to the cell nucleus.
  • Recent research indicates progress in addressing these multifaceted obstacles.

Conclusions:

  • Overcoming manufacturing, formulation, stability, and in vivo delivery challenges is crucial for the success of non-viral gene delivery systems.
  • Continued innovation is essential to translate the potential of nucleic acid therapeutics into viable commercial products.
  • Advancements in overcoming these barriers pave the way for broader applications of gene therapy.