[Isolation of regulation genes related with liver regeneration by representational difference analysis]

mRNA isolated from 2/3 partially hepatectomied rats was used as a tester for representational difference analysis (RDA). The subtracted tester cDNA was cloned into a T vector and a rat regeneration liver specific EST pool was constructed, which contained about 30000 independent clones. A sequence analysis of 52 clones randomly picked up from this pool indicated that the liver regeneration specific sequences were enriched, and the results of RNA blots revealed some novel genes in association with liver regeneration.