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Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body

Ivan Le Masson1, Cosmin Saveanu, Anne Chevalier

  • 1Service de Biochimie et de Génétique Moléculaire, Bât. 142, CEA/Saclay, F-91191 Gif-sur-Yvette, France.

Molecular Microbiology
|April 16, 2002
PubMed

Insights

Mps2 protein interacts with Spc24, a kinetochore component, aiding spindle pole body insertion into the nuclear envelope. This interaction is crucial for chromosome segregation during cell division.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Mps2 (monopolar spindle protein) is essential for spindle pole body (SPB) insertion into the nuclear envelope.
  • SPB duplication and insertion are critical processes in cell division.

Purpose of the Study:

  • To identify proteins interacting with Mps2.
  • To investigate the functional significance of the Mps2-Spc24 interaction in chromosome segregation.

Main Methods:

  • Yeast two-hybrid screening to identify Mps2 interacting partners.
  • Co-immunoprecipitation and in vitro binding assays to confirm protein interactions.
  • Analysis of thermosensitive spc24 mutants for defects in chromosome segregation and SPB duplication.

Main Results:

  • Identified Bbp1, Ynl107w, and Spc24 as Mps2 interacting proteins.
  • Verified the Mps2-Spc24 interaction in vivo and in vitro.
  • Confirmed interactions between Spc24 and Spc25/Ndc80.
  • Observed chromosome segregation defects in spc24 mutants, but not SPB duplication defects.

Conclusions:

  • The Mps2-Spc24 interaction likely contributes to the localization of kinetochore components to the SPB inner plaque.
  • Spc24, Spc25, and Ndc80 are peripheral kinetochore components vital for chromosome segregation.
  • This study elucidates a key interaction in the process of SPB insertion and chromosome segregation.

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