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Related Experiment Videos

Decrease in circulating hematopoietic progenitor cells by trapping in the pulmonary circulation.

T Watanabe1, T Kajiume, Y Takaue

  • 1Department of Pediatrics, University of Tokushima School of Medicine, Tokushima, Japan.

Cytotherapy
|April 16, 2002
PubMed
Summary

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Stem cells mobilized for transplantation can become trapped in the lungs, potentially reducing their effectiveness. This pulmonary trapping of peripheral blood stem cells (PBSC) was observed in patients undergoing mobilization.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Pulmonary Circulation

Background:

  • Stem cells infused intravenously encounter pulmonary capillaries first.
  • This initial encounter creates a potential for circulating stem and progenitor cells to be trapped in the lungs.

Purpose of the Study:

  • To investigate the extent of progenitor cell trapping in the pulmonary circulation.
  • To compare the number and cell-adhesion molecule (CAM) expression of progenitor cells in arterial and venous samples post-mobilization.

Main Methods:

  • Simultaneous sampling of radial arteries and central veins from 21 patients post-PBSC mobilization.
  • Quantification of progenitor cells (CD34(+) cells, CFU-GM, CD34(+)CD41(+) cells, CFU-meg) and CAM expression.

Main Results:

Related Experiment Videos

  • Reduced frequencies of all measured progenitor cell types in radial arteries compared to central veins (70-79%).
  • This reduction indicates significant pulmonary trapping of stem and progenitor cells.
  • No correlation found between CAM expression and the degree of pulmonary trapping.

Conclusions:

  • Pulmonary trapping of PBSC occurs during mobilization.
  • This trapping may negatively impact PBSC harvest and subsequent bone marrow trafficking.
  • Clinical significance is likely minimal if adequate cell numbers are infused.