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Updated: Oct 1, 2026
Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
Published on: November 1, 2018
[Effects of recombinant human leukemia inhibitor on the preimplantation mouse embryo development in vitro]
Guoyun Wang1, Xiaohui Deng, Huiqin Zhang
1Department of Obstetrics, Shandong Maternal and Child Health Hospital, Jinan 250014, China.
Objective:
To assess the effect of recombinant human leukemia inhibitory factor (rhLIF) on mouse embryo development in vitro.
Methods:
Mice were randomly divided to three groups, one in vivo control (group I) and two in vitro (group II and III). Mice were sacrificed at 116 - 120 hours (group I) and 44 - 48 hours (group II and III) subsequent human chorionic gonadotropin (hCG) injection. Two-cell embryos (group II and III) and blastocysts (group I) were obtained. Embryos in group II were cocultured with human tubal fluid (HTF) + 10% human serum and in group III with HTF + 10% human serum + rhLIF (1 000 U/ml). The number of embryo in different stage was recorded and compared.
Results:
Embryo in four, eight-cell and morula was noted in group II and III, 87.7% versus 91.2% and 75.0% versus 85.4% respectively. There was no significant difference. However, further embryo development to the blastocyst, expanded blastocyst, and hatching blastocyst in group II (48.1%, 32.1% and 18.4%) was lower than that in group III (82.3%, 59.7% and 36.3%). There was no difference between blastocyst in group I and group III (86.0% vs 82.3%).
Conclusion:
RhLIF does not provide obvious stimulation in early mouse embryo, however, rhLIF can promote the growth, differentiation, and hatching of preimplantion blastocyst.
