Related Experiment Videos
Yersinia pseudotuberculosis produces a cytotoxic necrotizing factor
Hank A Lockman1, Rebecca A Gillespie, Beth D Baker
1Children's Research Institute, Children's Hospital, Columbus, Ohio 43205-2696, USA. lockmanh@pediatrics.ohio-state.edu
Infection and Immunity
|April 16, 2002
Abstract:
Cell extracts from Yersinia pseudotuberculosis induced multinucleation in HEp-2 cells in a manner similar to the effect caused by Escherichia coli cytotoxic necrotizing factor (CNF). The activity was not dependent on the Yersinia 70-kb virulence plasmid, and the activity was not inhibited by antibodies capable of neutralizing E. coli CNF type 1. The nucleotide sequence of the Yersinia cnf gene was 65.1% identical to the E. coli cnf gene.