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Isolation of a SIR-like gene, SIR-T8, that is overexpressed in thyroid carcinoma cell lines and tissues
F de Nigris1, J Cerutti, C Morelli
1Servizio di Oncologia Sperimentale "E", Istituto per lo Studio e la Cura dei Tumori, Fondazione G. Pascale, 80131 Naples, Italy.
Abstract:
We used subtractive library screening to identify the changes that occur in gene expression during thyroid cell neoplastic transformation. Complementary DNA from normal thyroid cells (HTC 2) was subtracted from a complementary DNA library constructed from a human thyroid papillary carcinoma cell line. The library was screened for genes upregulated in human thyroid papillary carcinoma cell line cells, and several cDNA clones were isolated. One of these clones has a sirtuin core and high homology with the human silent information regulator protein family. This clone, designated "SIR-T8", was overexpressed in human thyroid carcinoma cell lines and tissues, but not in adenomas. The human SIR-T8 protein has a molecular weight of 39 kDa and is primarily located in the cytoplasm under the nuclear membrane. The SIR-T8 gene is located on chromosome 17q25-1.
Insights
Researchers identified SIR-T8, a gene overexpressed in thyroid carcinoma. This finding aids in understanding thyroid cancer development and may offer new diagnostic markers for neoplastic transformation.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Thyroid cell neoplastic transformation involves complex gene expression changes.
- Identifying specific upregulated genes is crucial for understanding cancer development.
Purpose of the Study:
- To identify genes upregulated during thyroid cell neoplastic transformation.
- To characterize a novel gene, SIR-T8, involved in thyroid carcinoma.
Main Methods:
- Subtractive library screening was employed using complementary DNA (cDNA) from normal thyroid cells and thyroid papillary carcinoma cells.
- Screening identified upregulated genes in cancer cells, leading to the isolation of several cDNA clones.
- One clone, SIR-T8, was further analyzed for its expression and characteristics.
Main Results:
- SIR-T8, homologous to the silent information regulator protein family, was identified and overexpressed in thyroid carcinoma cell lines and tissues.
- SIR-T8 was not found to be overexpressed in thyroid adenomas, distinguishing it from benign tumors.
- The SIR-T8 protein (39 kDa) is localized in the cytoplasm near the nuclear membrane, and its gene resides on chromosome 17q25-1.
Conclusions:
- SIR-T8 is a novel gene significantly overexpressed in thyroid carcinoma.
- Its specific upregulation in carcinoma, but not adenomas, suggests a role in neoplastic transformation.
- SIR-T8 may serve as a potential biomarker for thyroid cancer diagnosis.
