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Related Concept Videos

T Cell Activation and Clonal Selection01:22

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
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Related Experiment Video

Updated: May 5, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
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CD83 expression influences CD4+ T cell development in the thymus.

Yoko Fujimoto1, LiLi Tu, Ann S Miller

  • 1Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.

Cell
|April 17, 2002
PubMed
Summary
This summary is machine-generated.

CD83 is crucial for CD4+ T cell development in the thymus. Mice lacking CD83 show a significant reduction in mature CD4+ T cells, impacting immune responses.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • T lymphocyte development and lineage commitment in the thymus depend on intricate signaling pathways.
  • Thymic epithelial and dendritic cells play key roles in T cell maturation.

Purpose of the Study:

  • To investigate the role of the surface molecule CD83 in CD4+ T cell development within the thymus.
  • To determine the impact of CD83 deficiency on thymocyte differentiation and peripheral T cell populations.

Main Methods:

  • Utilized CD83-deficient (CD83-/-) mice to study T lymphocyte development.
  • Analyzed thymocyte populations and peripheral T cell subsets in wild-type and CD83-/- mice.
  • Performed cell transfer experiments using wild-type and CD83-/- thymocytes and bone marrow stem cells.

Main Results:

  • CD83 deficiency caused a specific developmental block in CD4+ single-positive thymocytes.
  • Peripheral CD4+ T cell numbers were reduced by 75%-90% in CD83-/- mice, mainly affecting naive T cells.
  • CD83 expression on thymic cells is essential for the differentiation of mature CD4+ T cells.

Conclusions:

  • CD83 is a critical regulatory molecule for CD4+ T cell development in the thymus.
  • The absence of CD83 leads to a profound defect in generating functional CD4+ T cells.
  • CD83 engagement is a necessary signal for proper T lymphocyte maturation.