Projections from the amygdaloid complex to the magnocellular cholinergic basal forebrain in rat

The amygdaloid complex has a key role in the modulation of behavioral responses in life-threatening situations, including the direction of attentional responses to sensory stimuli. The pathways from the amygdala to the basal forebrain cholinergic system, which projects to the cortex, are proposed to contribute to the modulation. To further explore the topography and postsynaptic targets of these pathways, we investigated the projections from the different divisions of the lateral, basal, accessory basal, and central nuclei of the amygdala to the cholinergic basal forebrain in rat using a sensitive anterograde tracer, Phaseolus vulgaris leucoagglutinin. The most substantial projections from the amygdala to the basal forebrain are directed to the ventrolateral and dorsomedial aspects of the substantia innominata and the fundus of the striatum. The heaviest projections originate in the capsular, lateral, and intermediate divisions of the central nucleus as well as in the magnocellular and parvicellular divisions of the basal nucleus. Light microscopic analysis of double-stained preparations revealed that the distribution of amygdaloid efferents and cholinergic neurons overlaps most prominently in the ventrolateral substantia innominata. Despite the fact that the central nucleus efferents and cholinergic elements overlap in the ventrolateral substantia innominata, electron microscopic analysis revealed, first, that the postsynaptic targets of the central nucleus efferents are non-cholinergic, probably GABAergic, neurons. Second, 80% of the synaptic contacts were symmetric. The present data extend previous observations showing that the different amygdaloid nuclei provide projections to the selective basal forebrain areas. Further, the central nucleus efferents modulate cholinergic neurons in the basal forebrain indirectly via the GABAergic interneurons.