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Effect of vancomycin on Staphylococcus epidermidis adherence to poly(methyl methacrylate) intraocular lenses
Taraprasad Das1, Savitri Sharma, Adiraju V Muralidhar
1Smt Kanuri Santhama Retina Vitreous Service, L.V. Prasad Eye Institute, Hyderabad, India. tpd@lvpeye.stph.net
Purpose:
To study the adherence of Staphylococcus epidermidis in variable loads (10(8) cfu/mL and 10(3) cfu/mL) to poly(methyl methacrylate) (PMMA) intraocular lenses (IOLs) and to evaluate the effect of vancomycin treatment on S epidermidis adherence to the IOL.
Setting:
L.V. Prasad Eye Institute, Hyderabad, India.
Methods:
The study was designed in 2 parts. Phase I: Twelve PMMA IOLs were soaked in a solution of S epidermidis, randomized to 10(8) cfu/mL or 10(3) cfu/mL. They were rinsed or rinsed and vortexed and cultured. Phase II: Twelve IOLs were treated with vancomycin (10 mg/mL), randomized to before and after treatment with S epidermidis.
Results:
Staphylococcus epidermidis adhered to all portions of the IOL with 10(8) cfu/mL and 10(3) cfu/mL bacterial loads. Treatment with vancomycin reduced S epidermidis adherence.
Conclusion:
Pretreatment of an IOL with vancomycin or a suitable antibiotic agent appears to reduce bacterial adherence to the IOL. Placement of a sustained-release device that could release an antibiotic agent over a specific period, reducing the incidence of postcataract endophthalmitis, should be studied.
Insights
Vancomycin treatment effectively reduced Staphylococcus epidermidis adherence to poly(methyl methacrylate) intraocular lenses (IOLs) in laboratory settings. Further research into sustained-release antibiotic devices may help prevent post-cataract infections.
Area of Science:
- Ophthalmology
- Microbiology
- Biomaterials Science
Background:
- Post-cataract endophthalmitis is a severe complication.
- Bacterial adherence to intraocular lenses (IOLs) contributes to infection.
- Staphylococcus epidermidis is a common pathogen in ocular infections.
Purpose of the Study:
- To investigate Staphylococcus epidermidis adherence to poly(methyl methacrylate) (PMMA) intraocular lenses (IOLs) at varying bacterial concentrations.
- To evaluate the efficacy of vancomycin treatment in reducing bacterial adherence to IOLs.
Main Methods:
- Two phases of experiments were conducted using PMMA IOLs.
- Phase I involved soaking IOLs in Staphylococcus epidermidis solutions (10^8 cfu/mL and 10^3 cfu/mL), followed by rinsing or vortexing.
- Phase II assessed the impact of vancomycin (10 mg/mL) treatment on bacterial adherence, applied before and after bacterial exposure.
Main Results:
- Staphylococcus epidermidis demonstrated adherence to all parts of the PMMA IOLs, irrespective of the initial bacterial load (10^8 cfu/mL and 10^3 cfu/mL).
- Vancomycin treatment significantly reduced the adherence of Staphylococcus epidermidis to the IOLs.
Conclusions:
- Pre-treatment of IOLs with vancomycin or other antibiotics can decrease bacterial adherence.
- Development and study of sustained-release antibiotic-eluting devices are recommended to minimize post-cataract endophthalmitis incidence.