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Cyclin D1 polymorphism and the susceptibility to NPC using DHPLC
Lin Deng1, Xiao-Rong Zhao, Kai Feng Pan
1Laboratory of Molecular Biology, Cancer Research Institute, Xiang-ya School of Medicine, Central South University, Changsha 410078, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|April 18, 2002
Summary
The cyclin D1 gene
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Cyclin D1 is a proto-oncogene implicated in various cancers.
- A common CCND1 A/G polymorphism affects mRNA transcripts and tumor phenotypes.
- Nasopharyngeal carcinoma (NPC) is a cancer where cyclin D1 deregulation is observed.
Purpose of the Study:
- To investigate the association between cyclin D1 genotypes and genetic susceptibility to sporadic nasopharyngeal carcinoma (NPC).
- To determine if CCND1 gene polymorphisms influence NPC risk in Southern Chinese populations.
Main Methods:
- Cyclin D1 genotyping using denaturing high performance liquid chromatography (DHPLC) and DNA sequencing.
- Analysis of PCR products from 84 NPC cases and 91 normal controls.
- Statistical analysis including Hardy-Weinberg equilibrium and chi-squared test.
Main Results:
- The AA genotype of CCND1 was significantly less frequent in NPC patients (20.24%) compared to controls (38.46%).
- GG and AG genotypes (GG + AG) were significantly more prevalent in the NPC group (chi-squared = 6.946, P corrected = 0.016, OR = 2.463).
Conclusions:
- The A/G polymorphism in the CCND1 gene is associated with susceptibility to nasopharyngeal carcinoma.
- GG and AG genotypes of CCND1 are linked to a higher risk of developing NPC in this population.