Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Virus protein assembly in microgravity.

D Chang1, A Paulsen, T C Johnson

  • 1BioServe Space Technologies, Kansas State University, Manhattan, KS 66506, USA.

Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of human papillomavirus infection and genotype distribution among male patients in a hospital in Shanghai City from 2022 to 2024].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025
Same author

Maternal immune memory and sex differences in living donor kidney transplant outcomes.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2025
Same author

Transarterial embolisation in the treatment of persistent haematuria in two dogs with lower urinary tract carcinoma.

The Journal of small animal practice·2025
Same author

Early antiviral use and supplemental oxygen decrease the risk of secondary bacterial infections: a multi-centre, nested, case-control study.

The Journal of hospital infection·2024
Same author

Active-reset protein sensors enable continuous in vivo monitoring of inflammation.

Science (New York, N.Y.)·2024
Same author

Phenotypes based on respiratory drive and effort to identify the risk factors when P0.1 fails to estimate ∆P<sub>ES</sub> in ventilated children.

Critical care (London, England)·2024

Polyomavirus VP1 protein self-assembles into capsid-like structures. Microgravity conditions yielded homogenous capsomeres, unlike ground controls which showed varied sizes and capsid assembly.

Area of Science:

  • Structural biology
  • Virology
  • Space biology

Background:

  • The polyomavirus capsid is composed of three proteins, with VP1 being the major component (75%).
  • VP1 protein subunits possess the ability to self-assemble into icosahedral capsid-like structures.
  • Understanding viral capsid assembly is crucial for developing antiviral therapies and nanotechnology.

Purpose of the Study:

  • To investigate the effect of microgravity on the self-assembly of polyomavirus VP1 protein.
  • To compare the structural characteristics of VP1 assemblies formed in microgravity versus ground-based conditions.

Main Methods:

  • VP1 protein was cloned, expressed, and purified.
  • Ground-based and microgravity (STS-37 space flight) experiments were conducted.
Keywords:
NASA Discipline Cell BiologyNon-NASA Center

Related Experiment Videos

  • Protein assembly was initiated by interfacing VP1 solution with a Ca+2 buffer (pH 5.0).
  • Samples were analyzed using electron microscopy after fixation.

    • Main Results:

    • Ground-based experiments showed VP1 assembly into capsid-like structures with heterogeneous capsomere sizes.
    • Microgravity-exposed samples exhibited homogenous-sized capsomeres but lacked capsid-like assembly.
    • Assembly initiation and termination processes were observed in both conditions.

    Conclusions:

    • Microgravity influences the self-assembly process of polyomavirus VP1 protein.
    • Homogenous capsomere formation in microgravity suggests altered assembly dynamics compared to ground conditions.
    • Further research is needed to elucidate the mechanisms behind microgravity-induced changes in viral protein assembly.