Electrical stimulation-induced alpha1- and alpha2-adrenoceptors-mediated contractions of isolated canine lymph nodes

The contractile effects of alpha-adrenoceptor agonists and field electrical stimulation were investigated pharmacologically in canine isolated tracheobronchial lymph nodes. Addition of noradrenaline (NA), phenylephrine (Phe) and UK14,304 caused dose-related contractions of the isolated lymph nodes. Pretreatment with prazosin (3 x 10(-9), 3 x 10(-8) and 3 x 10(-7) M) shifted the concentration-response curve for phenylephrine to the right, whereas yohimbine (3 x 10(-7) M) did not affect the contractile responses to phenylephrine. On the other hand, the concentration-response curve for UK 14,304 shifted to the right by treatment with yohimbine (3 x 10(-9), 3 x 10(-8) and 3 x 10(-7) M) but not by prazosin (3 x 10(-7) M). Field electrical stimulation (25 V, 0.7 ms, 16 Hz) produced contractile responses of the lymph nodes. The electrical stimulation-induced contractions were completely reduced by pretreatment with tetrodotoxin (TTX) (3 x 10(-7) M) or bretylium (10(-6) M). Furthermore, phentolamine (10(-8), 10(-7) and 10(-6) M), prazosin (3 x 10(-9) and 3 x 10(-8) M) and yohimbine (3 x 10(-8) and 3 x 10(-7) M) significantly reduced the electrical stimulation-induced contractions in the preparations. The present findings suggest that there are both alpha1- and alpha2-adrenoceptors located on the smooth muscle cells in canine tracheobronchial lymph nodes, and that the adrenoceptors-mediated excitatory mechanisms of adrenergic innervation exist in the lymph nodes.