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In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium
Yao-Yuan Hsieh1, Chich-Sheng Lin, Yu-Ling Sun
1Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
Purpose:
Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium.
Materials And Methods:
Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF-liposome (Group 1) and luciferase-liposome complexes (Group 2) were injected into their uterine lumen (Day 0). Endometrial LIF and luciferase expressions were detected by reverse transcription-polymerase chain reaction on Days 0-4 post gene transfer. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize the gene transfection.
Results:
LIF mRNA and luciferase activities reached the peak expression on Day 3. In Group 1, the ratios of LIF/GADPH on Days 1-4 were 0.414, 1.096, 1.162, and 0.782. In Group 2. LIF/GADPH on Days 1-4 were 0.24, 0.22, 0.35, and 0.32.
Conclusions:
Mouse endometrium could be effectively transfected with liposome-DNA mixtures. Endometrial LIF transfer via liposome may be effective in human.