Kinetic behaviour under defined assay conditions for bovine lens aldose reductase

Bovine lens aldose reductase, an enzyme important in sugar cataract formation, has been purified using a new procedure, and its optimum conditions of assay defined in both lens homogenates and purified preparations. Preliminary kinetic analysis shows that the degree of the rate equation is of a low order, and may be Michaelian under the standard conditions used.