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Stabilized lipid film based biosensor for atenolol
Dimitrios P Nikolelis1, Maria Mitrokotsa
1Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, 15771, Athens, Greece. nikolelis@chem.uoa.gr
Biosensors & Bioelectronics
|April 18, 2002
Summary
This study presents a new method for stabilizing lipid film biosensors for atenolol detection, enabling their use after air storage. These stabilized biosensors show reliable performance for repetitive measurements, paving the way for commercial applications.
Area of Science:
- Biomedical Engineering
- Materials Science
- Analytical Chemistry
Background:
- Lipid film biosensors offer potential for drug detection but often lack stability for practical use.
- Storage in air degrades the performance of traditional lipid-based sensing devices.
- Atenolol detection is crucial for cardiovascular health monitoring.
Purpose of the Study:
- To develop and characterize a stabilized lipid film biosensor for atenolol.
- To investigate the stability of lipid films on microporous supports after air storage.
- To compare the performance of stabilized lipid biosensors with freely suspended bilayer lipid membranes (BLMs).
Main Methods:
- Formation of polymerized lipid films on glass fiber microporous filters using methacrylic acid, ethylene glycol dimethacrylate, and 2,2'-azobis-(2-methylpropionitrile).
- Characterization of the stabilized lipid film biosensor's response to atenolol.
- Comparison of signal transduction (artificial ion gating events) with conventional BLMs.
Main Results:
- Stabilized lipid film biosensors demonstrated functionality after storage in air.
- The devices exhibited transient signals characteristic of artificial ion gating, similar to BLMs.
- Repetitive use was achieved with the stabilized lipid membrane biosensors.
Conclusions:
- The developed technique effectively stabilizes lipid film biosensors for air storage.
- Stabilized lipid biosensors provide a viable alternative to BLMs for repetitive sensing applications.
- This advancement facilitates the practical application and commercialization of lipid film-based chemical sensors.