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Related Experiment Videos

Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells.

Kathryn G Klemic1, James F Klemic, Mark A Reed

  • 1Department of Cellular and Molecular Physiology, School of Medicine, Yale University, 333 Cedar Street, New Haven, CT 06520, USA.

Biosensors & Bioelectronics
|April 18, 2002
PubMed
Summary

This study introduces poly(dimethylsiloxane) (PDMS) as a novel material for patch clamp electrodes, enabling high-resolution membrane current measurements. PDMS offers potential for high-throughput patch clamp recordings using planar electrode arrays.

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Area of Science:

  • Biophysics
  • Materials Science
  • Electrophysiology

Background:

  • The patch clamp technique is crucial for measuring cellular membrane currents at high resolution.
  • Traditional patch clamp electrodes are made of glass, which can be difficult to fabricate into specific shapes and may have limitations in high-throughput applications.

Purpose of the Study:

  • To investigate the use of poly(dimethylsiloxane) (PDMS), a silicone elastomer, for fabricating patch clamp electrodes.
  • To assess the suitability of PDMS electrodes for establishing high-resistance seals and performing patch clamp recordings.

Main Methods:

  • Fabrication of planar patch clamp electrodes using micromolded PDMS.
  • Modification of PDMS surface chemistry to mimic glass hydrophilicity.
  • Oxidation of the PDMS electrode surface.

Related Experiment Videos

  • Performing patch clamp recordings on Xenopus oocytes using PDMS electrodes.

    • Main Results:

    • PDMS electrodes were successfully fabricated with a shape mimicking glass micropipettes.
    • Altering PDMS surface chemistry allowed for the formation of high-resistance seals with cell membranes.
    • Patch clamp recordings were achieved on Xenopus oocytes using planar PDMS electrodes.
    • The results demonstrated the feasibility of using PDMS for patch clamp applications.

    Conclusions:

    • Poly(dimethylsiloxane) (PDMS) is a viable and promising material for patch clamp electrode fabrication.
    • PDMS electrodes offer advantages such as low dielectric loss and micromoldability.
    • The development of planar PDMS electrode arrays holds potential for advancing high-throughput patch clamp recording techniques.