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Related Experiment Videos

Rhythms in human bone marrow and blood cells.

Rune Smaaland1, Robert B Sothern, Ole D Laerum

  • 1Department of Oncology, Haukeland Hospital, University of Bergen, Norway.

Chronobiology International
|April 19, 2002
PubMed
Summary

Bone marrow (BM) cell proliferation and myeloid progenitor cells exhibit significant daily and yearly rhythms. Optimizing BM procedures based on these circadian rhythms can improve progenitor cell yield and treatment timing.

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Area of Science:

  • Hematology
  • Chronobiology

Background:

  • Circadian rhythms influence various physiological processes, including cell proliferation.
  • Understanding temporal variations in bone marrow cell activity is crucial for optimizing therapeutic interventions.

Purpose of the Study:

  • To investigate the circadian and circannual rhythms of bone marrow (BM) cell proliferation and progenitor cells in healthy individuals.
  • To explore the relationship between BM cell cycling and glutathione levels.
  • To assess the potential for timing BM procedures for improved yield and efficacy of treatments.

Main Methods:

  • 24-hour studies of bone marrow cells from healthy men.
  • Analysis of deoxyribonucleic acid (DNA) synthesis phase (S-phase) in total BM cells.
  • Assessment of colony-forming units--granulocyte/macrophage (CFU-GM) as a measure of myeloid progenitor cells.

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  • Measurement of BM glutathione content.
  • Evaluation of CD34+ progenitor stem cell yield.

    • Main Results:

    • Significant circadian rhythms were observed in BM cell DNA S-phase and CFU-GM, with peak activity around midday.
    • Both DNA S-phase and CFU-GM displayed circannual rhythms, peaking around August 12.
    • BM glutathione content peaked earlier in the day, preceding BM proliferative peaks.
    • Myeloid and erythroid precursor cells showed circadian variations and were positively correlated.
    • CD34+ progenitor stem cell yield also exhibited significant circadian variation.

    Conclusions:

    • Bone marrow cell cycling demonstrates distinct circadian and circannual rhythms, influencing sensitivity to growth factors and cytotoxic drugs.
    • Temporal synchrony in BM cell cycling suggests specific times for increased sensitivity.
    • Timing BM harvesting based on identified circadian rhythms may enhance progenitor cell yield.
    • Blood marker rhythms (e.g., white blood cells, neutrophils, lymphocytes, cortisol) can predict BM cell cycling peaks, enabling individualized timing of BM procedures.