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A pleiotropic regulatory mutation in lambda bacteriophage.

A Honigman, A Oppenheim, A B Oppenheim

    Molecular & General Genetics : MGG
    |January 1, 1975
    PubMed
    Summary
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    Lambda bacteriophage sar mutants exhibit restricted growth on specific hosts, indicating a role for cro and cII gene products in this inhibition. These sar mutations affect N and O gene expression and transcription.

    Area of Science:

    • Molecular Biology
    • Bacteriophage Genetics
    • Gene Regulation

    Background:

    • Lambda bacteriophage (λ) is a model system for studying gene regulation and lysogeny.
    • Understanding the intricate regulatory networks governing phage growth and host interaction is crucial.

    Purpose of the Study:

    • To isolate and characterize novel lambda bacteriophage mutants, termed lambdasar.
    • To elucidate the genetic basis and regulatory mechanisms underlying the observed growth restriction phenotype of lambdasar mutants.

    Main Methods:

    • Isolation and characterization of lambdasar mutants.
    • Genetic mapping of sar mutations within the origin (ori) region.
    • Complementation experiments to assess gene expression.
    • Transcription analyses in cro constitutive hosts.

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  • Phenotypic analysis of suppression of cy mutations.

    • Main Results:

    • Sar mutations confer a restriction phenotype on nonimmune, cro constitutive hosts, implicating cro and cII gene products.
    • Sar mutations map to the ori region and cause defects in N and O gene expression and transcription.
    • The Sar phenotype is suppressed by mutations affecting cro activity or by altering the immunity region.
    • Sar mutations suppress cy mutations in cis, requiring cII and cIII gene products, potentially by activating a new repressor synthesis promoter (pro).

    Conclusions:

    • The sar mutation plays a significant role in regulating lambda bacteriophage growth and gene expression.
    • Both cro and cII gene products are critical for the restriction phenotype observed in lambdasar mutants.
    • The sar mutation's ability to suppress cy mutations suggests a novel regulatory mechanism involving cII, cIII, and potentially a new promoter for repressor synthesis.