Variation in average unit chain length of glycogen in relation to developmental stage in Blastocladiella emersonii

Synchronous, single generations of Blastocladiella emersonii were grown along either the ordinary colorless or resistant sporangial plant pathways. Samples of cells were withdrawn at different developmental stages and glycogen was extracted, purified, debranched by isoamylase treatment, and its component unit chains separated by gel permeation chromatography. The elution profiles showed the distribution of unit chains. Average unit chain length was determined for plants at different developmental stages and shown to vary between 9 and 16. Some of these variations were correlated with other developmental events in the fungus.