Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Are we closer to developing threshold limit values for allergens in the workplace?

D Heederik1

  • 1Institute for Risk Assessment Sciences, Division of Environmental and Occupational Health, Universiteit Utrecht, Utrecht, the Netherlands. d.heederik@iras.uu.nl

Current Opinion in Allergy and Clinical Immunology
|April 20, 2002
PubMed
Summary

Immunoassays enable precise measurement of airborne protein allergens, aiding exposure assessments for workers like bakers and lab animal handlers. Further standardization is needed for regulatory standard setting.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial Effects of Livestock Farming on Human Infections With Shiga Toxin-Producing <i>Escherichia coli</i> O157 in Small but Densely Populated Regions: The Case of the Netherlands.

GeoHealth·2020
Same author

Respiratory diseases and allergy in farmers working with livestock: a EAACI position paper.

Clinical and translational allergy·2020
Same author

Atmospheric dispersion modelling of bioaerosols that are pathogenic to humans and livestock - A review to inform risk assessment studies.

Microbial risk analysis·2020
Same author

Stability of individual LPS-induced ex vivo cytokine release in a whole blood assay over a five-year interval.

Journal of immunological methods·2018
Same author

An approach to the asthma-protective farm effect by geocoding: Good farms and better farms.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2018
Same author

Challenges and Opportunities for Occupational Epidemiology in the Twenty-first Century.

Current environmental health reports·2017

Area of Science:

  • Occupational health
  • Immunology
  • Analytical chemistry

Background:

  • High molecular weight sensitizers, typically proteins, are common occupational allergens.
  • Exposure to these allergens can lead to adverse health effects in various work environments.
  • Accurate measurement of airborne allergen levels is crucial for risk assessment.

Purpose of the Study:

  • To highlight the utility of immunoassays in quantifying airborne protein allergens.
  • To evaluate the application of these measurements in understanding exposure-response relationships.
  • To discuss the potential for immunoassay use in occupational standard setting.

Main Methods:

  • Immunoassays were employed to measure airborne sensitizers.
  • Quantification was performed in the picogram and nanogram per cubic meter range.

Related Experiment Videos

  • Exposure data were collected for specific worker groups.

    • Main Results:

    • Immunoassays successfully facilitated the measurement of high molecular weight sensitizers.
    • Exposure-response relationships were evaluated for bakery workers exposed to wheat allergens and fungal alpha-amylase.
    • Similar evaluations were conducted for other occupational groups, such as laboratory animal workers.

    Conclusions:

    • Immunoassays are valuable tools for assessing occupational exposure to protein allergens.
    • The application of these methods for standard setting is currently limited but promising.
    • Rigorous standardization will be essential for future regulatory applications.