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Clarithromycin increases the release of heat shock protein B from Helicobacter pylori

T Tsuzuki1, K Ina, M Ohta

  • 1First Department of Internal Medicine, Nagoya University School of Medicine, Showa-ku, Nagoya, Japan.

Alimentary Pharmacology & Therapeutics
|April 23, 2002
PubMed
Abstract

Insights

Clarithromycin (CAM) may enhance the release of heat shock protein B (HspB) from Helicobacter pylori (H. pylori). This may boost the immune response against H. pylori and reduce gastric inflammation in patients.

Area of Science:

  • Microbiology
  • Immunology
  • Gastroenterology

Background:

  • Clarithromycin (CAM) may exert indirect effects on Helicobacter pylori (H. pylori) beyond growth inhibition.
  • Investigating lower CAM concentrations on H. pylori heat shock protein B (HspB) release and host immune response is crucial.

Purpose of the Study:

  • To examine the effects of sub-inhibitory concentrations of CAM on HspB release from H. pylori.
  • To assess changes in humoral immune response and gastric inflammation in patients undergoing H. pylori eradication therapy with CAM.

Main Methods:

  • H. pylori strains were cultured with varying CAM concentrations (2-500 ng/mL).
  • Protein expression was analyzed using 2D-electrophoresis and N-terminal sequencing.
  • Host immune response and histological gastritis were monitored in patients receiving H. pylori eradication therapy.

Main Results:

  • CAM (30-500 ng/mL) increased HspB release in all tested H. pylori clinical isolates.
  • Patients with failed CAM-based triple therapy showed increased anti-HspB IgG1/IgG2 antibodies.
  • These patients also exhibited reduced neutrophil infiltration and H. pylori colonization.

Conclusions:

  • CAM may induce a humoral immune response against H. pylori.
  • CAM might decrease gastric mucosal inflammation by up-regulating HspB release from H. pylori.

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