Related Experiment Video

Updated: Sep 30, 2026

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
12:16

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Published on: March 13, 2013

The novel tumour suppressor gene ING1 is overexpressed in human melanoma cell lines

E I Campos1, K-J J Cheung, A Murray

  • 1Department of Medicine, Division of Dermatology, Vancouver Hospital and Health Sciences Centre, University of British Columbia, Vancouver, BC, Canada.

The British Journal of Dermatology
|April 23, 2002
PubMed
Abstract

Insights

The inhibitor of growth 1 (ING1) gene is overexpressed in melanoma cell lines, suggesting a role in melanoma development. Mutations in ING1 are rare, indicating overexpression is the primary mechanism in these cell lines.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ultraviolet (UV) radiation is a known risk factor for melanoma, but the underlying genetic mechanisms are unclear.
  • The tumor suppressor gene inhibitor of growth 1 (ING1) inhibits cell growth and promotes apoptosis.
  • ING1 expression increases after UV irradiation and aids in DNA repair.

Purpose of the Study:

  • To investigate the role of ING1 in melanoma formation.
  • To analyze ING1 expression and mutation status in melanoma cell lines.

Main Methods:

  • Examined p33ING1 mRNA and protein expression in 14 melanoma cell lines.
  • Utilized single-strand conformation polymorphism (SSCP) and DNA sequencing to detect alterations in the ING1 gene.
  • Assessed ING1 gene polymorphism in healthy volunteers.

Main Results:

  • p33ING1 was overexpressed in melanoma cell lines compared to normal melanocytes.
  • Nucleotide alterations in the ING1 gene were identified in two melanoma cell lines (Sk-mel-24 and Sk-mel-110).
  • One alteration in Sk-mel-24 caused an amino acid change (Asn to Ser); other alterations were silent and unlikely due to polymorphism.

Conclusions:

  • ING1 is overexpressed in melanoma cell lines.
  • Mutations in ING1 occur infrequently in melanoma cell lines.
  • Overexpression, rather than mutation, appears to be the significant alteration of ING1 in melanoma.

More Related Videos

A Melanoma Patient-Derived Xenograft Model07:07

A Melanoma Patient-Derived Xenograft Model

Published on: May 20, 2019

Ex Vivo Culture of Circulating Tumor Cells in the Cerebral Spinal Fluid from Melanoma Patients to Study Melanoma-Associated Leptomeningeal Disease06:25

Ex Vivo Culture of Circulating Tumor Cells in the Cerebral Spinal Fluid from Melanoma Patients to Study Melanoma-Associated Leptomeningeal Disease

Published on: March 29, 2024

Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...