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Updated: Oct 1, 2026

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
Published on: March 17, 2018
Gastrin-releasing peptide, a bombesin-like neuropeptide, promotes cutaneous wound healing
Yuji Yamaguchi1, Ko Hosokawa, Yuka Nakatani
1Department of Dermatology, Osaka University Graduate School of Medicine, Suita-shi, Osaka, Japan.
Background:
Little is known about the effects of neuropeptides on wound healing.
Objective:
To investigate the effect of gastrin-releasing peptide (GRP), one of the bombesin-like neuropeptides, on wound healing.
Methods:
The effects of GRP on cultured keratinocyte proliferation and migration were measured by BrdU uptake and in vitro scratch assay, respectively. Various concentrations of GRP ointments (0, 10(-9), 10(-8), 10(-7), 10(-6) M) were topically applied to 1.0 mm wounds on porcine flanks.
Results:
GRP stimulated keratinocyte growth and locomotion in a dose-dependent manner. Topical administration of GRP accelerated macroscopic epidermal regeneration in a dose-dependent manner, as measured by planimetry. Histologic studies also showed that GRP promoted reepithelialization, including epidermal thickness as well as superficial skin coverage.
Conclusion:
Topical use of GRP may clinically accelerate wound healing of burns, injuries, chronic ulcers, and skin graft donor sites through the enhancement of keratinocyte growth and spreading.
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