Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence for a pre-restriction point Cdk3 activity.

Susan M Keezer1, David M Gilbert

  • 1Department of Biochemistry and Molecular Biology, S.U.N.Y. Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA.

Journal of Cellular Biochemistry
|April 23, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription elongation can be sufficient, but is not necessary, to advance replication timing.

EMBO reports·2026
Same author

Microhomology-mediated end joining acts directly on replication forks to repair single-ended double-strand breaks.

Molecular cell·2026
Same author

Microhomology-mediated end joining acts directly on replication forks to repair single-ended double strand breaks.

bioRxiv : the preprint server for biology·2026
Same author

An integrated view of the structure and function of the human 4D nucleome.

Nature·2025
Same author

Autosomal Allelic Inactivation: Variable Replication and Dosage Sensitivity.

bioRxiv : the preprint server for biology·2025
Same author

RIF1 orchestrates a multi-step restoration of post-replicative H3K9me3.

bioRxiv : the preprint server for biology·2025
Same journal

Tricarboxylic Acid (TCA) Cycle Enzyme Alpha-Ketoglutarate Dehydrogenase (KGDH) as a Nexus for the Regulation of Macrophage Polarization.

Journal of cellular biochemistry·2026
Same journal

Zinc Finger Proteins as Regulators of Organ Fibrosis.

Journal of cellular biochemistry·2026
Same journal

Intrinsic Disorder Status in Human Proteins Interacting With SARS-CoV-2 Proteins: Insights From Five Years of Translational Research.

Journal of cellular biochemistry·2026
Same journal

The Effect of Protein Tagging on Aggregation and Phase Separation.

Journal of cellular biochemistry·2026
Same journal

TRIM32 Alleviates the Inflammation in Spinal Cord Injury Progression Through Inducing the Ubiquitination Degradation of TLR4.

Journal of cellular biochemistry·2026
Same journal

Bedaquiline Binding at the Leading Site of Mycobacterium tuberculosis ATP Synthase Induces Distinct Structural and Dynamic Changes.

Journal of cellular biochemistry·2026
See all related articles

Cyclin-dependent kinase 3 (cdk3) activity peaks before the restriction point in the cell cycle. This pre-restriction point activity requires new protein synthesis and the breakdown of an inhibitor.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cyclin-dependent kinases (cdks) regulate cell cycle progression.
  • The precise role of cdk3 in the G1-phase remains incompletely understood.
  • Identifying key regulators of cell cycle transitions is crucial for understanding cell proliferation.

Purpose of the Study:

  • To investigate the activity and regulation of cyclin-dependent kinase 3 (cdk3) during the G1-phase of the cell cycle.
  • To determine the temporal window of cdk3 activation relative to the restriction point.
  • To elucidate the molecular mechanisms controlling cdk3 activity.

Main Methods:

  • Immunoprecipitation of cdk3 from Chinese Hamster Ovary (CHO) fibroblasts.
  • Assay of histone H1 kinase activity in anti-cdk3 immunoprecipitates.

Related Experiment Videos

  • Treatment with roscovitine, MG132 (proteasome inhibitor), and cycloheximide (protein synthesis inhibitor).

    • Main Results:

    • Histone H1 kinase activity associated with cdk3 peaked 2-3 hours before the G1 restriction point.
    • In vitro cdk3 activity was inhibited by roscovitine, consistent with inhibition of cdks 1, 2, and 5.
    • Early G1-phase cdk3 activation was reduced by MG132 and cycloheximide, indicating dependence on protein synthesis and degradation.

    Conclusions:

    • A distinct pre-restriction point cdk3 activity exists during early G1-phase.
    • This activity is regulated by the synthesis of a regulatory subunit and the degradation of an inhibitory protein.
    • cdk3 plays a critical, temporally regulated role in the G1-phase transition.