Evaluation of a single-step multiplex RT-PCR for influenza virus type and subtype detection in respiratory samples

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Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
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Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV

Published on: November 10, 2023

Saibal K Poddar1, Rowena Espina, David P Schnurr

  • 1Pediatric Pharmacology Research Unit, Department of Pediatrics, University of California-San Diego, La Jolla, California 92093-0808, USA. spoddar@ucsd.edu

Journal of Clinical Laboratory Analysis
|April 23, 2002
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...