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Related Experiment Videos

Highly robust stainless steel tips as microelectrospray emitters.

Yasushi Ishihama1, Hiroyuki Katayama, Naoki Asakawa

  • 1Analytical Research Laboratories, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. yasushi@bmb.sdu.dk

Rapid Communications in Mass Spectrometry : RCM
|April 23, 2002
PubMed
Summary

New stainless steel spray tips for sheathless microelectrospray ionization (microESI) offer significantly enhanced sensitivity and stability. These durable tips are ideal for routine proteomic and pharmaceutical analyses using capillary electrophoresis/mass spectrometry (CE/MS) and LC/MS.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Materials Science

Background:

  • Sheathless microelectrospray ionization (microESI) requires robust and sensitive emitters.
  • Conventional ion spray systems can lack the sensitivity needed for complex analyses.
  • Development of stable, high-performance emitters is crucial for advanced analytical techniques.

Purpose of the Study:

  • To develop and optimize tapered stainless steel spray tips for sheathless microESI.
  • To evaluate the sensitivity, stability, and applicability of the developed tips.
  • To assess the suitability of the tips for integrated analytical systems like CE/MS and LC/MS.

Main Methods:

  • Fabrication of tapered stainless steel spray tips using electropolishing and burr removal.

Related Experiment Videos

  • Optimization of the electropolishing technique for tip fabrication.
  • Testing the microESI emitter performance at a flow rate of 20 nL/min.

    • Main Results:

    • A stable electrospray ionization (ESI) spray was achieved at 20 nL/min.
    • The microESI system demonstrated sensitivity nearly two orders of magnitude greater than conventional systems.
    • The stainless steel tips exhibited high stability, functioning effectively for over 1000 hours.

    Conclusions:

    • Optimized tapered stainless steel spray tips provide a stable and highly sensitive emitter for sheathless microESI.
    • These tips are robust and suitable for long-term use in demanding analytical applications.
    • The developed tips are compatible with sheathless capillary electrophoresis/mass spectrometry (CE/MS) and capillary liquid chromatography/mass spectrometry (LC/MS) for proteomic and pharmaceutical analyses.