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Related Experiment Videos

Alpha-2 adrenoceptor activity affects propofol-induced sleep time.

Tetsuya Kushikata1, Kazuyoshi Hirota, Hitoshi Yoshida

  • 1Department of Anesthesiology, University of Hirosaki School of Medicine, Japan. tkush@df6.so-net.ne.jp

Anesthesia and Analgesia
|April 26, 2002
PubMed
Summary

The alpha(2) adrenoceptor plays a role in anesthesia. This study shows that the alpha(2) agonist clonidine increased propofol-induced sleep time, while the alpha(2) antagonist yohimbine decreased it, affecting norepinephrine release.

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Area of Science:

  • Anesthesiology
  • Neuroscience
  • Pharmacology

Background:

  • Alpha(2) adrenoceptor activity is implicated in the mechanisms of anesthesia.
  • Clonidine (an agonist) and yohimbine (an antagonist) affect barbiturate-induced sleep times.

Purpose of the Study:

  • To investigate the effects of clonidine and yohimbine on propofol-induced sleep time in rats.
  • To correlate these effects with changes in prefrontal cortex norepinephrine release.

Main Methods:

  • Male Wistar rats were administered varying doses of yohimbine or clonidine, followed by propofol.
  • Sleep duration and prefrontal cortex norepinephrine levels (via microdialysis) were measured.

Main Results:

  • Yohimbine (1 mg/kg) decreased propofol sleep time by ~30% and increased norepinephrine by ~240%.

Related Experiment Videos

  • Clonidine (300 and 30 µg/kg) increased sleep time by ~260% and ~170%, respectively, while decreasing norepinephrine by ~60%.

    • Conclusions:

    • Alpha(2) adrenoceptor activation (clonidine) increases propofol-induced sleep time.
    • Alpha(2) adrenoceptor inhibition (yohimbine) decreases propofol-induced sleep time.
    • The alpha(2) receptor is a valuable target for understanding anesthetic mechanisms.