Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nerve growth factor promotes olfactory axonal elongation.

Lee V Martin1, Steven Weston, Adrian K West

  • 1Discipline of Anatomy and Physiology, University of Tasmania, Hobart, Australia 7001.

Neuroreport
|April 26, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IPEM topical report: guidance for the use of linac manufacturer integrated quality control.

Physics in medicine and biology·2025
Same author

IPEM topical report: results of a 2022 UK survey on the use of linac manufacturer integrated quality control (MIQC).

Physics in medicine and biology·2023
Same author

The influence of metallothionein treatment and treadmill running exercise on disease onset and survival in SOD1<sup>G93A</sup> amyotrophic lateral sclerosis mice.

The European journal of neuroscience·2020
Same author

Aurora kinase B regulates axonal outgrowth and regeneration in the spinal motor neurons of developing zebrafish.

Cellular and molecular life sciences : CMLS·2018
Same author

Profiling cellular and inflammatory changes in the airway wall of mild to moderate COPD.

Respirology (Carlton, Vic.)·2017
Same author

The main rhinovirus respiratory tract adhesion site (ICAM-1) is upregulated in smokers and patients with chronic airflow limitation (CAL).

Respiratory research·2017

Nerve growth factor (NGF) significantly enhances olfactory axonal elongation in developing rats. The olfactory bulb secretes NGF, which promotes the growth of olfactory axons during development.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Olfactory system development involves complex axonal guidance cues.
  • Nerve growth factor (NGF) is a key signaling molecule in neuronal development.
  • Understanding factors promoting olfactory axon growth is crucial for regenerative medicine.

Purpose of the Study:

  • To investigate the role of NGF in olfactory axonal elongation.
  • To determine if olfactory bulb explants secrete NGF.
  • To elucidate the mechanism of NGF-mediated olfactory axon growth.

Main Methods:

  • Explant culture of E14 rat olfactory epithelium.
  • Application of exogenous NGF (50 ng/ml).
  • Immunostaining for TrkA receptors and S-100.

Related Experiment Videos

  • Immunoassay to detect NGF secretion from olfactory bulb explants.

    • Main Results:

    • Exogenous NGF significantly enhanced olfactory neurite elongation (p = 0.025).
    • Neurites expressed active TrkA receptors, indicating NGF signaling.
    • S-100-positive ensheathing cells were present.
    • E14 presumptive olfactory bulb explants secreted NGF into the culture medium.

    Conclusions:

    • NGF promotes olfactory axonal elongation during development.
    • The olfactory bulb is a source of NGF for developing olfactory axons.
    • NGF-TrkA signaling facilitates olfactory axon growth in vivo.