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Updated: Jul 24, 2026

FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
The assignment of PRKCI to bovine chromosome 1q34-->q36 by FISH suggests a new assignment to human chromosome 3
M De Donato1, D S Gallagher, S K Davis
1Departamento de Biomedicina, IIBCA, Universidad de Oriente, Cumana, Venezuela. marcosdedonato@yahoo.com
Abstract:
Two bovine BAC clones were identified by PCR as containing the bovine gene PRKCI. Both clones were assigned by FISH to bands q34-->q36 on BTA1. The sequence information derived from genomic DNA and from both clones was identical and showed a high degree of homology to human PRKCI (HSAXq21.3, 95.5% homology), and mouse Prkcl (MMU3, 13.8 cM, 87.6% homology) and rat Prkcl (88.8% homology). This assignment could suggest a disruption of the synteny conservation of mammalian X-linked genes, but most likely suggests a misassignment of this gene to the human X.
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