Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Excluded volume effects in gene stretching.

Pui-Man Lam1

  • 1Physics Department, Southern University, Baton Rouge, LA 70813. pmlam@phys.subr.edu

Biopolymers
|April 30, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extension, torque, and supercoiling in single, stretched, and twisted DNA molecules.

The Journal of chemical physics·2015
Same author

Role of chain entropy in an analytic model of protein binding in single-DNA stretching experiments.

Physical review. E, Statistical, nonlinear, and soft matter physics·2011
Same author

Discrete persistent-chain model for protein binding on DNA.

Physical review. E, Statistical, nonlinear, and soft matter physics·2011
Same author

Adsorption of externally stretched two-dimensional flexible and semiflexible polymers near an attractive wall.

Physical review. E, Statistical, nonlinear, and soft matter physics·2009
Same author

Driven translocation of a polynucleotide chain through a nanopore: a continuous time Monte Carlo study.

Physical review. E, Statistical, nonlinear, and soft matter physics·2006
Same author

Unzipping DNA from the condensed globule state-effects of unraveling.

Biopolymers·2005

Excluded volume effects alter DNA stretching, causing non-linear force-extension relationships. A freely jointed chain model accurately predicts this behavior, with stronger effects observed in two-dimensional systems.

Area of Science:

  • Polymer physics
  • Biophysics
  • Statistical mechanics

Background:

  • Understanding single DNA molecule behavior in solution is crucial for molecular biology and nanotechnology.
  • Excluded volume interactions, arising from the finite size of polymer segments, significantly influence polymer conformations and properties.
  • Previous models often simplify these interactions, potentially limiting their accuracy in describing DNA stretching.

Purpose of the Study:

  • To investigate the impact of excluded volume effects on the stretching of single DNA molecules in solution.
  • To develop and validate a model that accurately describes DNA extension under varying forces.
  • To compare excluded volume effects in two-dimensional versus three-dimensional systems.

Main Methods:

  • Theoretical analysis of a freely jointed chain model incorporating excluded volume interactions.

Related Experiment Videos

  • Derivation of the force-extension relationship, particularly for small applied forces.
  • Comparison of model predictions with experimental data for lambda-phage DNA stretching.

    • Main Results:

    • For small forces (F), DNA extension (h) is non-linearly proportional to F raised to the power of gamma, where gamma = (1 - nu)/nu.
    • The universal correlation length exponent (nu) characterizes this non-linear behavior.
    • The freely jointed chain model, with adjusted segment length, accurately fits experimental lambda-phage DNA data across the entire force range.

    Conclusions:

    • Excluded volume effects are critical for accurately modeling single DNA stretching, leading to deviations from linear elasticity.
    • The proposed freely jointed chain model provides a robust framework for understanding DNA elasticity.
    • Excluded volume effects are more pronounced in two dimensions, suggesting potential for novel experiments in reduced dimensions.