Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MCP-1 expression in endotoxin-induced uveitis.

Nadine Tuaillon1, De Fen Shen, Ravi B Berger

  • 1Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bldg. 10 Room 10-N-103, 10 Center Drive, Bethesda, MD 20892-1857, USA.

Investigative Ophthalmology & Visual Science
|May 1, 2002
PubMed
Summary

Monocyte chemoattractant protein-1 (MCP-1) is crucial for endotoxin-induced uveitis (EIU). Reducing MCP-1 significantly lessens ocular inflammation, suggesting it as a therapeutic target for acute anterior uveitis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Different connectivity patterns of MRI-negative drug-resistant epilepsy and drug-sensitive epilepsy in cerebellar and subcortical-cortical regions.

Neurobiology of disease·2026
Same author

A human case of Q fever associated with cat in China: a neglected risk factor of Q fever.

One health outlook·2026
Same author

FSEA: Incorporating domain-specific prior knowledge for few-shot weed detection.

Plant phenomics (Washington, D.C.)·2025
Same author

Over 4 decades, French children teach about LCH.

Blood·2025
Same author

Author Correction: Molecular profiling of 888 pediatric tumors informs future precision trials and data-sharing initiatives in pediatric cancer.

Nature communications·2024
Same author

Molecular profiling of 888 pediatric tumors informs future precision trials and data-sharing initiatives in pediatric cancer.

Nature communications·2024

Area of Science:

  • Ophthalmology
  • Immunology
  • Inflammation Research

Background:

  • Monocyte chemoattractant protein (MCP)-1 (CCL-2) is a key chemokine involved in monocyte recruitment.
  • Acute anterior uveitis is a common and potentially vision-threatening ocular inflammatory condition.

Purpose of the Study:

  • To investigate the role of MCP-1 in the development of endotoxin-induced uveitis (EIU), a model for acute anterior uveitis.
  • To assess the therapeutic potential of targeting MCP-1 for ocular inflammation.

Main Methods:

  • Endotoxin-induced uveitis (EIU) was induced in MCP-1 knockout mice and wild-type controls using lipopolysaccharide (LPS).
  • Recombinant MCP-1 was administered to MCP-1 knockout mice to assess its necessity for EIU induction.
  • Ocular inflammation was evaluated through histological analysis and gene expression profiling (RT-PCR) of inflammatory markers.

Related Experiment Videos

Main Results:

  • EIU development and ocular inflammatory cell infiltration were significantly reduced in MCP-1 knockout mice compared to wild-type controls.
  • Intraocular interferon-gamma (IFN-gamma) transcription was elevated in MCP-1 knockout mice during EIU.
  • Re-administration of recombinant MCP-1 restored EIU in knockout mice, confirming MCP-1's essential role.

Conclusions:

  • MCP-1 plays a critical role in the pathogenesis of acute anterior uveitis.
  • Targeting MCP-1 presents a promising therapeutic strategy for managing acute anterior uveitis.