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E2F-3B is a physiological target of cyclin A.

Yiwen He¹, W Douglas Cress

¹Department of Biochemistry and Molecular Biology, University of South Florida, College of Medicine, Tampa 33612, USA.

The Journal of Biological Chemistry

|May 1, 2002

Summary

Cyclin A binds to and regulates the activity of E2F-3B, a cell cycle transcription factor. This finding challenges previous predictions and identifies E2F-3B as a physiological target of cyclin A.

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Area of Science:

Cell cycle regulation
Molecular biology
Gene transcription

Background:

The E2F family of transcription factors is crucial for the G(1)/S cell cycle transition.
Cyclin A/Cdk2 complexes regulate E2F-1, -2, and -3A activity, leading to degradation.
E2F-3B, an alternative splice variant of E2F-3A, was predicted to lack cyclin A binding.

Purpose of the Study:

To investigate the interaction between cyclin A and E2F-3B.
To determine if E2F-3B is a functional target of cyclin A.

Main Methods:

In vitro binding assays with bacterially expressed proteins.
In vivo binding assays in mammalian cells.
Co-expression studies to assess transcriptional activity.
Analysis of protein expression in synchronized cells.

Main Results:

Cyclin A directly binds to E2F-3B both in vitro and in vivo.
Co-expression of cyclin A reduces E2F-3B transcriptional activity.
E2F-3B protein levels decrease when cyclin A levels rise in synchronized cells.

Conclusions:

E2F-3B interacts with cyclin A, contradicting prior predictions.
E2F-3B is a physiological target of cyclin A, suggesting a role in cell cycle regulation.

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