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Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs
D Jonas1, M Speck, F D Daschner
1National Reference Center of Hygiene, Institute of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, Hugstetter Strasse 55, 79106 Freiburg, Germany. djonas@IUK3.UKL.uni-Freiburg.de
Journal of Clinical Microbiology
|May 1, 2002
Abstract:
A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA femB PCR with prior enrichment in selective broth was tested for 439 swabs. PCR identified 36 MRSA-positive samples, in concordance with conventional methods.