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Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
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Published on: July 16, 2011

Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo

Thi My Anh Neildez-Nguyen1, Henri Wajcman, Michael C Marden

  • 1Institut National de la Santé et de la Recherche Médicale U417, Hôpital Saint-Antoine, Paris, France.

Nature Biotechnology
|May 1, 2002
PubMed

New sources of red blood cells (RBCs) would improve the transfusion capacity of blood centers. Our objective was to generate cells for transfusion by inducing a massive proliferation of hematopoietic stem and progenitor cells, followed by terminal erythroid differentiation. We describe here a procedure for amplifying hematopoietic stem cells (HSCs) from human cord blood (CB) by the sequential application of specific combinations of growth factors in a serum-free culture medium. The procedure allowed the ex vivo expansion of CD34+ progenitor and stem cells into a pure erythroid precursor population. When injected into nonobese diabetic, severe combined immunodeficient (NOD/SCID) mice, the erythroid cells were capable of proliferation and terminal differentiation into mature enucleated RBCs. The approach may eventually be useful in clinical transfusion applications.

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