Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunomagnetic separation reagents as markers in electron microscopy.

Phyllis J Fisher1, Margaret J Springett, Allan B Dietz

  • 1Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First St., S.W., Rochester, MN 55905, USA. fisher.phyllis@mayo.edu

Journal of Immunological Methods
|May 2, 2002
PubMed
Summary

Immunomagnetic reagents, used for cell isolation, effectively bind to target cells for electron microscopy. These reagents facilitate the capture and concentration of rare cells, enabling detailed ultrastructural visualization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraarterial Autologous Mesenchymal Stem Cell Therapy for Diabetic Kidney Disease.

Kidney international reports·2025
Same author

Periadventitial delivery of mesenchymal stem cells improves vascular remodeling and maturation in arteriovenous fistulas.

Science translational medicine·2025
Same author

Using artificial intelligence to improve cell therapy assays: automated quantitative image analysis of cells on matrices.

Tissue & cell·2025
Same author

Summary of a Consensus Conference on the Management of Highly Sensitized Multiorgan Transplant Candidates.

Mayo Clinic proceedings·2025
Same author

Phase I study of pembrolizumab in combination with ibrutinib for the treatment of unresectable or metastatic melanoma.

Frontiers in immunology·2025
Same author

Fellowship training for cellular therapy laboratory directors.

Cytotherapy·2025

Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • Immunomagnetic cell isolation is established but its utility for electron microscopy (EM) is unevaluated.
  • Commercial antibodies conjugated to magnetic particles (iron-dextran) are available for cell isolation.

Purpose of the Study:

  • To evaluate the use of immunomagnetic reagents for electron microscopy.
  • To assess the association of immunomagnetic reagents with isolated cells.
  • To determine the suitability of immunomagnetic reagents for capturing rare cells for EM.

Main Methods:

  • Isolation of T cells and monocytes/macrophages from human peripheral blood mononuclear cells using immunomagnetic adsorption.
  • Analysis of immunomagnetic reagent association with cell membranes via electron microscopy.

Related Experiment Videos

  • Comparison of flow cytometry and EM for quantifying T cell binding using CD4-specific reagents.

    • Main Results:

    • CD14-positive cells retained magnetic beads on the plasma membrane, while live cells internalized them.
    • Flow cytometry and EM yielded indistinguishable results for CD4+ T cell quantification.
    • Immunomagnetic reagents remained associated with target cells, allowing secondary labeling with colloidal gold.

    Conclusions:

    • Immunomagnetic reagents are suitable for cell isolation and subsequent electron microscopy.
    • These reagents enable the purification and concentration of rare cells for ultrastructural studies.
    • The availability of diverse immunomagnetic reagents expands possibilities for cellular ultrastructure visualization.