Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High pressure static fluorescence to study macromolecular structure-function.

Kangcheng Ruan1, Claude Balny

  • 1Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai, PR China.

Biochimica Et Biophysica Acta
|May 2, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.

Cell research·2017
Same author

Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK.

PLoS pathogens·2017
Same author

A CRISPR-based approach for targeted DNA demethylation.

Cell discovery·2016
Same author

Profiling human protein degradome delineates cellular responses to proteasomal inhibition and reveals a feedback mechanism in regulating proteasome homeostasis.

Cell research·2014
Same author

A smartphone controlled handheld microfluidic liquid handling system.

Lab on a chip·2014
Same author

ZIP-seq: genome-wide mapping of trinucleotide repeats at single-base resolution.

Journal of molecular cell biology·2013
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

High pressure fluorescence methods reveal molecular structures and enzymatic behaviors. This technique analyzes intrinsic and extrinsic probes, offering insights into reactions under extreme conditions.

Area of Science:

  • Biophysical Chemistry
  • Spectroscopy
  • Biochemistry

Background:

  • Fluorescence spectroscopy is a powerful tool for molecular analysis.
  • Understanding biological processes under high pressure is crucial for various scientific fields.
  • High pressure can significantly alter molecular structures and reaction dynamics.

Purpose of the Study:

  • To describe the high pressure static fluorescence method.
  • To analyze the potential of fluorescence probes for structural characterization.
  • To investigate enzymatic reactions under high pressure using fluorescence.

Main Methods:

  • Utilizing high pressure static fluorescence spectroscopy.
  • Employing intrinsic and extrinsic fluorescence probes.

Related Experiment Videos

  • Applying fluorescence polarization techniques.

    • Main Results:

    • Demonstrated the utility of high pressure fluorescence for structural insights.
    • Showcased the application in studying enzymatic reactions under pressure.
    • Addressed spectroscopic challenges in data interpretation.

    Conclusions:

    • High pressure fluorescence is a valuable technique for structural and dynamic studies.
    • Fluorescence probes offer significant potential for characterizing biomolecules under pressure.
    • Further research is needed to refine data interpretation in high pressure fluorescence spectroscopy.