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Updated: Jul 15, 2026

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
Published on: April 6, 2011
Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) activate protein
J Z Nowak1, A Dejda, J B Zawilska
1Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
Abstract:
Pituitary adenylate cyclase-activating polypeptide (PACAP38) and vasoactive intestinal peptide (VIP) were tested for their ability to influence protein kinase C (PKC) activity in the chick cerebral cortical slices. Thirty minutes incubation of the chick tissue with PACAP38 (0.1-1 microM) or VIP (0.3-3 microM) produced significant and concentration-dependent changes in PKC activity. Both peptides enhanced the enzyme activity in cell membrane preparation, and decreased it in cytosol preparation obtained from cerebral cortical slices. These changes in PKC activity suggest that PACAP and VIP are capable of activating this enzyme in cerebral cortex of chick.
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