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Published on: November 21, 2014

Effect of CD14 promoter polymorphism and H. pylori infection and its clinical outcomes on circulating CD14

J Karhukorpi1, Y Yan, S Niemela

  • 1Department of Medical Microbiology, University of Oulu, Finland. jari.karhukorpi@oulu.fi

Clinical and Experimental Immunology
|May 3, 2002
PubMed

Insights

Serum soluble CD14 (sCD14) levels are influenced by genetic variations in the CD14 gene promoter and Helicobacter pylori infection. Both factors, along with gastric mucosal lesions, contribute to elevated sCD14 levels.

Area of Science:

  • Immunology
  • Genetics
  • Gastroenterology

Background:

  • CD14 is a key pattern recognition receptor involved in innate immunity.
  • Soluble CD14 (sCD14) circulates in serum and its levels can be affected by genetic and environmental factors.
  • Lipopolysaccharide (LPS) is a well-known ligand for CD14.

Purpose of the Study:

  • To investigate the impact of CD14 gene promoter polymorphism (-159C/T) and Helicobacter pylori infection on serum sCD14 levels.
  • To compare sCD14 levels in dyspeptic patients and healthy controls.
  • To identify other factors influencing sCD14 levels in dyspeptic patients.

Main Methods:

  • Serum sCD14 levels were measured using enzyme immunoassay (EIA).
  • CD14 genotyping was performed using PCR.
  • Helicobacter pylori infection was detected via serology, culture, or PCR.
  • Patient groups included dyspeptic individuals undergoing gastroscopy and healthy staff members.

Main Results:

  • Subjects with the T allele (CT or TT genotype) of the CD14 promoter polymorphism had elevated sCD14 levels compared to CC genotype.
  • H. pylori-positive individuals showed a trend towards higher sCD14 levels.
  • Dyspeptic patients with gastric ulcers, erosions, or duodenal ulcers had significantly higher sCD14 than those with normal findings or gastritis.
  • NSAID use was also associated with increased sCD14.

Conclusions:

  • Both genetic factors (CD14 promoter polymorphism) and environmental factors (H. pylori infection, mucosal lesions) significantly impact serum sCD14 levels.
  • sCD14 may serve as a potential biomarker for certain gastrointestinal conditions and infections.
  • Further research is warranted to explore the clinical implications of these findings.

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