Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromatin remodeling in nuclear cloning.

Paul A Wade1, Nobuaki Kikyo

  • 1Department of Pathology, Emory University, Atlanta, GA, USA.

European Journal of Biochemistry
|May 3, 2002
PubMed
Summary

Nuclear cloning uses somatic cell nuclei to create new animals, revealing oocyte cytoplasm

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methionine Deprivation-induced Reprogramming of Hepatic Rhythms Is Mediated by Glucocorticoid Receptor.

bioRxiv : the preprint server for biology·2026
Same author

Circadian regulator PER1 inhibits osteoclastogenesis by activating inflammatory genes.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same author

Circadian regulators PER1 and PER2 regulate osteoclastogenesis by balancing competing activities of innate immunity genes.

bioRxiv : the preprint server for biology·2026
Same author

Examining the Roles of m<sup>6</sup>A Sites in mRNA Using the Luciferase Gene Fused With Mutated RRACH Motifs.

Bio-protocol·2025
Same author

The circadian regulator PER1 inhibits osteoclastogenesis by activating inflammatory genes.

bioRxiv : the preprint server for biology·2025
Same author

A novel mutant allele of Mta3 in the mouse: genetic analysis of roles in immunity and androgen biology.

G3 (Bethesda, Md.)·2025

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Epigenetics

Background:

  • Nuclear cloning creates new animals by transferring somatic nuclei into oocytes.
  • Mammalian cloning successes suggest oocyte cytoplasm has potent reprogramming factors.
  • Cell differentiation is regulated by chromatin structure and transcriptional activity.

Purpose of the Study:

  • To explore the reprogramming activities within oocyte cytoplasm.
  • To understand the role of chromatin remodeling in nuclear reprogramming.
  • To investigate molecular mechanisms of nuclear dedifferentiation and redifferentiation.

Main Methods:

  • Somatic nucleus injection into unfertilized oocytes.
  • Analysis of chromatin remodeling processes.
  • Investigating modifications of chromatin elements (protein binding, histone acetylation/methylation, DNA methylation).

Main Results:

  • Oocyte cytoplasm possesses significant reprogramming capacity.
  • Chromatin remodeling is central to nuclear reprogramming.
  • Specific chromatin modifications are key to dedifferentiation and redifferentiation.

Conclusions:

  • Oocyte cytoplasm can erase cell differentiation memory.
  • Chromatin remodeling is crucial for reversing differentiated cell states.
  • Further studies on chromatin modifications will elucidate reprogramming mechanisms.

Related Experiment Videos