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Updated: Jul 7, 2026

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
10:41

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Published on: January 3, 2012

Polyclonal antibodies to glutathione S-transferase--verotoxin subunit a fusion proteins neutralize verotoxins.

P H M Leung1, J S M Peiris, W W S Ng

  • 1Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Special Administrative Region, People's Republic of China.

Clinical and Diagnostic Laboratory Immunology
|May 3, 2002
PubMed
Summary
This summary is machine-generated.

Researchers developed specific polyclonal antibodies against verotoxin-1 (VT1) and VT2 fusion proteins. These antibodies effectively neutralize homologous toxins, showing promise for diagnosing VT-producing E. coli infections.

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Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Published on: September 10, 2018

Area of Science:

  • Microbiology
  • Immunology
  • Molecular Biology

Background:

  • Verotoxins (VT1 and VT2) produced by E. coli are potent toxins responsible for severe gastrointestinal illness.
  • Developing specific antibodies for toxin neutralization is crucial for diagnostic and therapeutic applications.

Purpose of the Study:

  • To produce and characterize polyclonal antibodies targeting the A1 subunits of verotoxin-1 (VT1) and VT2.
  • To evaluate the toxin-neutralizing capabilities of these antibodies against various VT-producing E. coli strains.

Main Methods:

  • Cloning of VT1 and VT2 A1 subunits into a GST fusion protein expression vector (pGEX-4T-2).
  • Production of polyclonal antibodies in rabbits using purified GST-VT1A1 and GST-VT2A1 fusion proteins.
  • Assaying antibody neutralization activity against active toxins from 45 VTEC strains.

Main Results:

  • Polyclonal antibodies demonstrated high neutralizing activity against their homologous toxins (VT1 and VT2).
  • Anti-GST-VT1A1 antibodies neutralized VT1 with 76.7% efficiency, while anti-GST-VT2A1 antibodies neutralized VT2 with 82.5% efficiency.
  • Cross-neutralization was observed for a VT2 variant, suggesting sequence homology.

Conclusions:

  • This study reports the successful production of polyclonal antibodies from GST-VT fusion proteins with specific toxin-neutralizing activity.
  • These antibodies represent a valuable tool for the immunological diagnosis of infections caused by VT-producing E. coli (VTEC).