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Updated: Aug 7, 2026
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Published on: November 7, 2018
Development, evaluation, and application of lateral-flow immunoassay (immunochromatography) for detection of
Yousif Al-Yousif1, Joe Anderson, Cindy Chard-Bergstrom
1Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506, USA.
Abstract:
A lateral-flow immunoassay (LFT) was developed to detect bovine rotavirus in fecal samples. Using samples (n = 74) from diarrheic calves, a comparison of the LFT with a commercial latex agglutination test (LAT) and transmission electron microscopy (EM) was conducted. When EM was used as the reference method, initial studies of 29 samples indicated 70 and 80% sensitivities of the LFT and LAT, respectively, with both being 100% specific. When the LAT was the reference test, the LFT was 75% sensitive and 91% specific. Additional specimens (n = 45) were tested by the LFT and LAT alone, and results were identical for both methods.
