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Updated: Aug 12, 2026
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Screening and characterization of human phage antibody to hepatitis virus C NS5A antigen]
1Gene Therapy Research Center, Institute of Infectious Diseases, The 302nd Hospital of PLA, Beijing 100039, China.
Objective:
To screen and characterize human phage antibody against hepatitis C virus (HCV)NS5A antigen.
Methods:
The recombinant phages were panned by recombinant HCV NS5A antigen which was coated in a microtiter plate; after five rounds of biopanning, 35 clones were obtained and demonstrated specific to NS5A antigen. The specific binding capacity of the ScFv antibody to HCV NS5A antigen was determined by ELISA.
Results:
HCV NS5A phage antibody had a specific combination capacity with hepatitis C virus NS5A antigen. The DNA sequence data showed that the ScFv gene was composed of 789 bp and codes for a peptide of 262 amino acid residues.
Conclusions:
Human single chain antibody to hepatitis C virus NS5A antigen has been identified by means of the phage display technology.
