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[HIV genomic mutation on antiretroviral therapy]
1Department of Infectious Diseases, the First Clinic Hospital, Beijing Medical University, 100034 Beijing, China.
Background:
To study the characteristics of HIV genomic mutation on antiretroviral therapy.
Methods:
HIV DNA and HIV RNA were detected by using Taq-PCR, RT-PCR, LD-PCR, hybridization, and oligonucleotide 32P- probes against gag, tat, nef, LTRU3, etc. RESULYS: The HIV RNA load in serum declined significantly, CD4 lymphocyte count increased, copies of gag region of HIV DNA decreased, and full-length HIV DNA disappeared. Bands showing gag segments hybridized with 32P-probe appeared negative or less clear in some cases after antiretroviral therapy. However, the regions hybridized with probes Xho-nef and LTR U5 were apparently enhanced and some drawbands were seen.
Conclusions:
HIV gene may experiece obvious mutation on antiretroviral therapy.