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[Molecular epidemiological characterization of rabies virus isolated from Guangxi]
1Centers of Disease Control and Prevention of Guangxi, Nanning 530021,China.
Background:
To analyze the genotype of rabies virus isolated from different areas in Guangxi at different time.
Methods:
mRNA of 8 Guangxi isolates, 1 Anhui isolates and 10 isolates from different countries of rabies virus were amplified by reverse transcription polymerse chain reaction (TR-PCR),and the products were sequenced. Phylogenetic analysis was carried out by phylip program.
Results:
Phylogenetic analysis showed that 8 Guangxi isolates were related regionally, the homologic difference was lower than 2% among the isolates of the same area; higher than 15% among the isolates of two different areas.8 Guangxi isolates belonged to genotype A1, A2 a and A2 b; 10 chb of Guangxi isolates and thbkrb 2123 and thdg 2933 of Thailand isolates belonged to genotype A2 b, while Guangxi isolates were different from isolates of USA, Mexico and South Africa, which all belonging to genotype B.
Conclusions:
There are at least three groups of rabies virus based on limited sequencing of N gene in Guangxi.