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[Analyzing partial nucleotide and amino acid sequences of Chinese TTV isolates]
1Department of Clinical Laboratory, Eastern Hepatobiliary Hospital, The Second Military Medical University, Shanghai 200438, China.
Background:
To investigate the prevalence of transfusion transmitted virus (TTV) and its sequence characterization in China.
Methods:
TTV DNA was amplified by Semi-Nested-PCR, and the positive products were sequenced directly.
Results:
The homology of partial nucleotide and amino acid sequences of 7 Chinese TTV isolates were 64.7%-98.4% and 62.7%-96.4% respectively when compared with Japanese isolate. Among 7 Chinese TTV isolates, the homology of partial nucleotide and amino acid sequences were 63.5%-98.4% and 60.2%-96.4% respectively. They differently belong to two genotypes and two subgenotypes.
Conclusions:
There exists TTV in China, and there might be several genotypes of Chinese TTV isolates.